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Our studies are directed toward understanding the molecular biology of lentiviruses, in particular that of the major structural proteins, the viral nucleic acids and the role of the cell in the life cycle of the virus. We initiated an investigation of the mechanism of interaction of host and virus by expression of gag and env from the mammalian cell line COS.

This has led to the identification of a cellular translation factor that enhances the translation of viral RNA in vitro. This factor appears to also function in vivo and is involved in the generation of the gag and env polyprotein precursors. We are also developing a detailed molecular model of the env proteins in the membrane of the virus.

The role of the cell in the viral life cycle has been examined by insertion of the gene for the viral envelope glycoprotein env into an expression vector for the gene for a marker protein neo. Insertion of this marker gene into HIV-1, the feline leukemia virus and two strains of SIV allowed selection for insertion of the env gene in both orientations.

Since env has multiple functions, these insertion mutants can be used to study the effect of altering env function on viral infectivity and pathogenicity. Our studies have also focused on the regulation of the LTR promoter in the early phase of infection.

The transcription factors involved in the regulation of LTR and other promoters have been studied and shown to include host and virus-encoded products. Their sequence specificities for binding have been determined by oligonucleotide footprinting. Windows 8. Photoshop downloading for pc. Leave A Reply Cancel Reply. Next Post Photoshop downloading for pc. You might also like More from author.

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